How a Real-Time PCR System Works: An Introduction

If you’re still wondering what a real-time PCR system is, this article is for you. It introduces the topic and shares key points about how it works.

What is the Real-Time PCR System?

A real-time PCR system is a laboratory instrument that uses DNA sequencing to identify the location and sequence of specific genes or pieces of DNA. In other words, it’s a powerful tool for genetically analyzing samples. The process works like this: First, the sample (usually a small piece of tissue) is put into a PCR machine, amplifying the gene(s) you want to study. Then, the PCR machine sequences the amplified gene(s) and prints out the results.

The Benefits of a Real-Time PCR System

A real-time PCR system can be incredibly valuable for researchers and scientists. Several benefits of using a real-time PCR system include rapidly and accurately detecting and diagnosing disease. A real-time PCR system is also versatile, allowing researchers to perform various experiments.

Previously, scientists would have to wait several hours or days for a traditional PCR system results. However, with a Real-Time PCR System, results can be seen within minutes. This makes it much easier for scientists to decide how to proceed with their research quickly.

A real-time PCR system consists of several key components: the sample undergoes pre-incubation with the first gene probe in a reaction buffer; the temperature of the reaction is then gradually increased over several minutes to allow the probe to hybridize to its target sequence; after the target sequence has been amplified, the temperature is lowered again, and the probe is washed away; finally, the entire reaction is analyzed by either an electrophoresis gel or an optical detector. By regulating these key parameters – such as the temperature and duration of each cycle – researchers can generate accurate data that can be used to identify specific genes or proteins in their samples.


This article discusses what a real-time PCR system is, how it works, and some of the benefits that can be gained from using one. Modern real-time PCR systems are incredibly versatile and can be used for various purposes, such as identifying specific genes or proteins in samples, detecting infections or diseases, or monitoring environmental pollutants.

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